Part:BBa_K3036005:Design

Arabinose-induced suicide switch

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 65
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Arabinose-induced suicide switch is characterized in a system where the mazF (BBa_K302033) gene encoding toxin is put under the control of L-arabinose induced promoter pBAD (BBa_K206000). As is shown in Fig.1, the cell number of experimental groups show a significant decrease upon induction, which indicates the arabinose-induced suicide switch works upon induction of 1.25μM/L and 2.5μM/L L-arabinose.

Source

This part comes from Escherichia.coli DH5alpha.